Journal: Translational Neuroscience

Article Title: Effects of enriched environment on the expression of β-amyloid and transport-related proteins LRP1 and RAGE in chronic sleep-deprived mice

doi: 10.1515/tnsci-2022-0301

Figure Lengend Snippet: Effect of EE on RAGE expression in chronic sleep-deprived mice. Note: (a) prefrontal cortex and hippocampal RAGE protein bands in each group; (b and c) analysis of grayscale values of prefrontal cortex and hippocampal RAGE/GAPDH in each group; (d) immunofluorescence staining of prefrontal cortex RAGE (green), CD31 (red), and DAPI (blue) in each group, magnification scale bar = 100 μm, other scale bar = 20 μm; (e) immunofluorescence staining of prefrontal cortex and hippocampal RAGE (red), CD31 (green), and DAPI (blue) in each group, magnification scale bar = 50 μm, other scale bar = 20 μm; (f) percentage of CD31-RAGE co-localization area of prefrontal cortex stretching CD31 area in each group; and (g) percentage of CD31-RAGE co-localization area of hippocampal stretching CD31 area in each group ( n = 3, **** P < 0.001).

Article Snippet: The following primary antibodies were used: Rabbit anti-mouse LRP1 primary antibody (ab92544, 1:1,000; Abcam), Rabbit anti-mouse RAGE primary antibody (ab3611, 1:1,000; Abcam), and Rabbit anti-mouse GAPDH primary antibody (AP0063, 1:5,000; Bioworld).

Techniques: Expressing, Immunofluorescence, Staining

Journal: Basic Research in Cardiology

Article Title: Renal denervation reduces atrial remodeling in hypertensive rats with metabolic syndrome

doi: 10.1007/s00395-022-00943-6

Figure Lengend Snippet: A Representative fluorescence microscopy for RAGE (stained red by TRITC), atrial tissue autofluorescence (green) and nuclei (stained blue by DAPI) in left atria of control rats (left panel), SHR (first middle panel), SHRob (second middle panel) and SHRobRDN rats (right panel) ( n = 3 each group). Magnification 20x. Scale bar 50 µm . B Representative Western blot (upper panel) and quantification of left atrial (LA) RAGE (lower panel) and C sRAGE in left atrial homogenates from normotensive Ctr ( n = 9), SHR ( n = 8), SHRob ( n = 8) and SHRobRDN ( n = 8). RAGE and sRAGE in arbitrary units (AU) normalized to GAPDH. *p < 0.05 versus Ctr; §p < 0.05 versus SHR; #p < 0.05 versus SHRob

Article Snippet: Sections were then incubated overnight at 4 °C and incubated the following day for additional 2 h at 37 °C with the primary antibody diluted 1:300 (Abcam; ab37647, rabbit polyclonal to RAGE), followed by incubation with the corresponding secondary antibody (TRITC-conjugated donkey anti-rabbit IgG, 1:50 dilution (Jackson ImmunoResearch) at 37 °C for 90 min. 1×PBS buffer containing 0.1% Tween 20 was used for the necessary washing steps.

Techniques: Fluorescence, Microscopy, Staining, Control, Western Blot

Journal: Journal of Ocular Pharmacology and Therapeutics

Article Title: HMGB1 Antagonist, Box A, Reduces TLR4, RAGE, and Inflammatory Cytokines in the Cornea of P. aeruginosa -Infected Mice

doi: 10.1089/jop.2018.0073

Figure Lengend Snippet: Nucleotide Sequence of the Specific Primers Used for Polymerase Chain Reaction Amplification

Article Snippet: After blocking for 1 h in 5% MTBST (TBS containing 0.05% Tween 20 and 5% nonfat milk), membranes were probed with primary antibodies: rabbit anti-mouse TLR4 (1:80; Abcam Cambridge, MA) and rabbit anti-mouse RAGE (1:1,000; Abcam) in 3% BSA TBST overnight at 4°C.

Techniques: Sequencing, Polymerase Chain Reaction

Journal: Journal of Ocular Pharmacology and Therapeutics

Article Title: HMGB1 Antagonist, Box A, Reduces TLR4, RAGE, and Inflammatory Cytokines in the Cornea of P. aeruginosa -Infected Mice

doi: 10.1089/jop.2018.0073

Figure Lengend Snippet: Immunohistochemistry for HMGB1, TLR4, RAGE, and TNF-α in human cornea. Immunostaining of normal and Pseudomonas aeruginosa-infected corneas from human patients showed expression of HMBG1 (B), TLR4 (D), RAGE (F), and TNF-α (H) in corneas infected with P. aeruginosa when compared with similarly processed normal cornea (A, C, E, G). HMGB1, high mobility group box 1.

Article Snippet: After blocking for 1 h in 5% MTBST (TBS containing 0.05% Tween 20 and 5% nonfat milk), membranes were probed with primary antibodies: rabbit anti-mouse TLR4 (1:80; Abcam Cambridge, MA) and rabbit anti-mouse RAGE (1:1,000; Abcam) in 3% BSA TBST overnight at 4°C.

Techniques: Immunohistochemistry, Immunostaining, Infection, Expressing

Journal: Journal of Ocular Pharmacology and Therapeutics

Article Title: HMGB1 Antagonist, Box A, Reduces TLR4, RAGE, and Inflammatory Cytokines in the Cornea of P. aeruginosa -Infected Mice

doi: 10.1089/jop.2018.0073

Figure Lengend Snippet: RT-PCR and western blot for TLR4 and RAGE. Box A treatment significantly reduced corneal mRNA expression of TLR4 (A) and RAGE (B) at 5 days p.i. with no difference detected between groups in the uninfected, normal (N) cornea. Western blot and determination of relative protein expression by IDV analysis showed that Box A treatment reduced protein expression for TLR4 (C, E) and RAGE (D, F) in normal cornea and at 5 day p.i. No difference between treatment groups was seen at 3 days p.i. for either TLR4 or RAGE. An unpaired, 2-tailed Student's t-test determined significance for RT-PCR and protein expression data. P < 0.05 was considered significant; data are shown as mean ± SEM. IDV, integrated density value; RT-PCR, real-time polymerase chain reaction.

Article Snippet: After blocking for 1 h in 5% MTBST (TBS containing 0.05% Tween 20 and 5% nonfat milk), membranes were probed with primary antibodies: rabbit anti-mouse TLR4 (1:80; Abcam Cambridge, MA) and rabbit anti-mouse RAGE (1:1,000; Abcam) in 3% BSA TBST overnight at 4°C.

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Expressing, Real-time Polymerase Chain Reaction

Journal: Journal of Ocular Pharmacology and Therapeutics

Article Title: HMGB1 Antagonist, Box A, Reduces TLR4, RAGE, and Inflammatory Cytokines in the Cornea of P. aeruginosa -Infected Mice

doi: 10.1089/jop.2018.0073

Figure Lengend Snippet: Immunohistochemistry for HMGB1 and TLR4 or RAGE. Dual immunostaining of infected corneas from Box A versus PBS-treated mice showed a modest pattern of co-labeling (yellow) of HMBG1 (red) with TLR4 (green) at 3 (A, B) and 5 (E, F) days p.i. only in the PBS-treated group. In contrast Box A treatment resulted in little to no colocalization at 3 (C,D) or 5 (G,H) days p.i. A similar pattern of co-localization (yellow) was seen for HMGB1 and RAGE at 3 days p.i. (I–L), but co-localization of HMGB1 and RAGE at 5 days p.i, was seen only in the PBS-treated corneas (M, N), but not in the Box A treated group (O,P). Magnification: 230 × (A, C, E, G, I, K, M, O), magnification: 460 × (B, D, F, H, J, L, N, P).

Article Snippet: After blocking for 1 h in 5% MTBST (TBS containing 0.05% Tween 20 and 5% nonfat milk), membranes were probed with primary antibodies: rabbit anti-mouse TLR4 (1:80; Abcam Cambridge, MA) and rabbit anti-mouse RAGE (1:1,000; Abcam) in 3% BSA TBST overnight at 4°C.

Techniques: Immunohistochemistry, Immunostaining, Infection, Labeling